The main function is to produce ATP but there are functions not related to ATP are intimately involved with most major metabolic pathways used by a cell to build , operate and recycle their molecular components . Cells can not even make RNA and DNA they need to grow and function without mitochondria. The building blocks of DNA and RNA are purines and pyrimidines . Mitochondria contain the rate limiting enzymes of pyrimidine biosynthesis ( dihydroorotate dehydrogenase ) and the synthesis of heme ( d - amino levulinic acid synthetase ) necessary to produce hemoglobin . In the liver , mitochondria are specialized to detoxify ammonia in the urea cycle . Mitochondria for metabolism of cholesterol , for the synthesis of estrogen and testosterone , for metabolism of neurotransmitter , and to the production of free radicals and detoxification are also required.

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